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Of 49 bacterial isolates interfered with bioluminescence production under the conditions specified in the direct cell-cell assays. Chemical extracts were generated for the "active" isolates and tested in a disc diffusion assay at 500 g disc against both V. harveyi BB120 and C. violaceum ATCC 12472 for chemical activity. The extracts of 28 isolates inhibited bioluminescence only, while five were able to additionally inhibit violaceum production. 23% n 49 ; of our active isolates tested positive for the presence of a lactonase gene AiiA ; , suggesting that enzymatic degradation of homoserine lactone autoinducers may be a contributing mechanism for quorum sensing inhibition. This study suggests that non-toxic interactions may be a common phenomenon in microbe-microbe competition in the marine environment. P-036M: RHODIOLA CRENULATA ROOT EXTRACT CONTAINS POTENT ANTIANGIOGENIC COMPONENTS BEYOND GALLIC ACID Zhijun Liu1, Frank L. Greenway2, Eugene A. Woltering3, Dong Liu1, Ying Yu2, Josh Schwimer3, Peng Nan4, and Yang Zhong4. 1 LSU Agricultural Center, Baton Rouge, LA 70803 USA; 2Louisiana State University Pennington Biomedical Research Center, Baton Rouge, LA 70808; 3Louisiana State University Health Sciences Center, New Orleans, LA 70112 USA; 4Fudan University, Shanghai, China. Rhodiola crenulata is a precious medicinal herb in the Qinghai-Tibet Plateau in China. Traditional use focuses on its endurance properties in a region of high elevation, strong radiation, and thin air. Reportedly, this plant contains salidroside and is a rich source of polyphenols such as gallic acid, crenulatin, p-tyrosol, p-hydroxyphenethyl alcohol 1-O- 3-n- 6"-O-galloyl ; -glucopyranoside, 1, 2, 3, rhodionin, and rhodiosin. Due to our knowledge of gallic acid as an antiangiogenic agent, we predicted this plant was antiangiogenic if gallic acid concentrations were present in sufficient amount to trigger an in vitro response. In testing this hypothesis, a novel human tissue-based placentas veins and adipose ; angiogenesis model was used. Rhodiola crenulata root extract was prepared with a 70% ethanol maceration method. At 1 mg ml concentration, this extract completely blocked angiogenesis, which confirmed our hypothesis based on gallic acid. However, questions remained as to whether non-gallic acid components also contributed to the overall anti-angiogenic activity, and more importantly whether more potent pharmacophores of gallic acid were present in the extract. To answer these questions, bioactivity directed fractionation and isolation was performed. It was found that both gallic acid and gallic acid-free fractions were active. Fractionation to separate the active compounds was successful and the simplified resultant fractions are better candidates for future isolations of active compounds, characterization of which will allow potency comparisons with gallic acid. P-037M: INFLUENCE OF VARIOUS CULTURE CONDITIONS ON THE GROWTH OF EUCOMMIA ULMOIDES HAIRY ROOTS Xiaojun Wu, Dong Liu, Zhijun Liu Medicinal Plant Lab, School of Renewable Natural Resources, Louisiana State University Agricultural Center, Baton Rouge, LA 70803, USA Established hairy roots of Eucommia produce desired secondary compounds. Biomass and concentrations are two determinants of compounds yield. This study was carried out to investigate the effects of several culture conditions on the growth of Eucommia ulmoides hairy roots. Results showed that light, initial pH of medium, and culture volume did not have remarkable effect on the growth of hairy roots, while other factors, including medium type, medium strength, sucrose concentration, auxin type and concentration influenced the growth significantly. 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