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Sumycin
Manufactured by Guatemala Laboratorios Richam Internacional, S.A. for Merck S.A. Guatemala do. do. Alcon Laboratories, USA Inc. Distributed by McNeil Consumer & Specialty Pharmaceuticals DIVISION OF MCNEIL-PPC, INC. USA Product of USA ; do. State University of Campinas, Campinas, Sao Paulo, Brazil ~ The tartrate-resistant acid phosphatases TRAPs ; are a class of metalloenzymes that catalyze the hydrolysis of various phosphate esters under acid reaction conditions. These enzymes can be used as biomarker for several diseases, including bone resorption. The purpose of this work was to determine the effect of hydrogen peroxide H2O2 ; and in the TRAP from human blood serum and a correlation with iron, since this enzyme contains two iron atoms at its active site [Fe III-FeII ; ]. The peripheric blood from healthy volunteers was collected and diluted in physiological solution 1: 6 ; . The enzyme activity was determined using p-nitrophenylphosphate as substrate 5mM ; and hepes buffer 100mM ; , pH 7.4. After 30 minutes the reaction was stopped with NaOH 1.0M ; , and p-nitrophenol was measured at 405nm. The TRAP was inhibited 50% by H2O2 60mM ; , and this effect was prevented in the presence of GSH 20mM ; , phenantrolin 10 mM ; and uric acid 5 mM ; . Despite the TRAP sensibility to H2O2, the enzyme was not inhibited by a typical protein tyrosine phosphatase inhibitor, pervanadate, which acts as oxidant. Since TRAP has no SH-residue in the active site, our results suggest a possible participation of this enzyme in the Fenton reaction in the presence of H2O2. Uric acid acts as hydroxyl scavenger, therefore, the enzyme activity protection observed in the presence of this compound confirmed that the TRAP catalyses the generation of reactive oxygen species. They usually disappear soon after the sumycin medication is stopped.
Editor-in-Chief Daniel B. Carr, MD Internal Medicine, Endocrinology, Anesthesiology USA.
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