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F-2 F-4 F-5 F-6 F-7 F-8 - F-10 F-11 - F-52 S-1 S-2 and verapamil. Anti-tumour effects. Utilizing a fusion receptor of the yeast Gal4DNA binding domain joined to the hinge region and ligand binding domain of the human PPAR in combination with a Gal4-driven luciferase reporter gene, cotransfected into Cos7 cells, we tested sage and rosemary extracts prepared with 80% aqueous ethanol for possible PPAR activation. This revealed that both extracts are capable of selectively activating Gal4-PPAR fusion receptor, in a concentration-dependent manner, with EC50 values of 22.8 8.4 mg L and 33.7 7.3 mg L for rosemary and sage, respectively. Subsequent analysis of the characteristic constituents revealed the phenolic diterpene compounds carnosol, present in both herbs, and carnosic acid to be active principles of these extracts, showing EC50 values of 41.2 5.9 M and 19.6 2.0 M, respectively. Thus it can be concluded that the glucose lowering effect reported recently for rosemary may be attributed to PPAR activation. Moreover, our observations may also explain the anti-inflammatory and antiproliferative effects of both compounds published previously. Georg Thieme Verlag KG Stuttgart. 729. Isolation and antimalarial activity of alkaloids from Pseudoxandra cuspidata - Roumy V., Fabre N., Souard F. et al. [Dr. N. Fabre, Laboratoire Pharmacochimie des Substances Naturelles et Pharmacophores Redox, Universit Toulouse 3 Paul Sabatier, e Faculte des Sciences Pharmaceutiques, 31062 Toulouse Cedex 9, France] - PLANTA MED. 2006 72 10 ; - summ in ENGL A novel and very unusual azaanthracene alkaloid, 1-aza-7, 8, 9, ; and a new diastereoisomer of the bis-benzylisoquinoline alkaloid rodiasine, 1S, 1'R-rodiasine 2 ; , as well as the alkaloids 0-methyl-punjabine 3 ; and O-methylmoschatoline 4 ; have been isolated from Pseudoxandra cuspidata bark, used in French Guiana as an antimalarial. Their structures were elucidated by spectroscopic analyses, especially 2D-NMR techniques ADEQUATE and NOESY ; . We found that the antimalarial activity of this bark was mostly due to bis-benzylisoquiaoline 1S, 1'R-rodiasine 2 ; IC50 1 M ; also displaying a low cytotoxicity. Georg Thieme Verlag KC Stuttgart. 730. The effect of cicerfuran, an arylbenzofuran from Cicer bijugum, and related benzofurans and stilbenes on Leishmania aethiopica, L. tropica and L. major - Getti G.T.M., Aslam S.N., Humber D.P. et al. [G.T.M. Getti, School of Health and Bioscience, University of East London, Stratford Campus, Romford Road, London E15 4LZ, United Kingdom] - PLANTA MED. 2006 72 10 ; - summ in ENGL The effect of 3 arylbenzofurans and 7 stilbenes on the growth of Leishmania parasites and human monocytes was evaluated. Promastigotes from cultures of L. aethiopica, L. major and L. tropica were tested in the exponential phase of growth. All compounds were active at concentrations of 100 g mL within 6 hours. The 2hydroxylstibene showed activity at a concentration 1 g mL, with an LD50 of 3-5 g mL after 48 hours of incubation. The most active compounds: cicerfuran, 2-hydroxy-2 -methyl-4 5 - methylenedioxystilbene, 2-hydroxy-2 -methoxy-4 , 5 - methylenedioxystilbene and 2-hydroxystilbene had even stronger activity against the temperature-induced amastigotes of L. aethiopica, with the latter having the highest relative potency against all three species. Leishmanicidal activity seemed to be associated with the level of oxygen substitution in each compound. The ratio between leishmanicidal activity on promastigotes and toxicity to human cells suggested that the compounds could be considered as leishmanicidal drug leads. Georg Thieme Verlag KG Stuttgart. 731. Endothelium-independent relaxation of aorta rings by two stilbenoids from the orchids Scaphyglottis livida - Estrada-Soto S., L pez-Guerrero J.J., Villalobos-Molina R. and Mata R. [R. o Mata, Departamento de Farmacia, Facultad de Quimica, Universi dad Nacional Aut noma de M xico, M xico, D. F. 04510, Mexico] o e e - FITOTERAPIA 2006 77 3 ; - summ in ENGL Gigantol 1 ; and 3, 7-dihydroxy-2, 4-dimethoxyphenanthrene ; from the orchid Scaphyglottis livida induced a significant concentration-dependent relaxation of the contractions evoked by noradrenaline NA ; in endothelium-intact and denuded rat aorta rings. Incubation with NG -nitro-l-arginine methyl ester l-NAME, 1 10- 5 M ; or methylene blue MB, 1 10- 7 M ; significantly reduced the relaxation induced by the stilbenoids 1 and 2. The Section 30 vol 138.2. 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